PP-ODS2 column needs to be washed. If the standard protocol as outlined in the user manual doesn’t solve the problem, proceed to wash the column as follows: 1. 30 min water in the reverse direction at half the normal flow rate. 2. 1 hr with 0.1% phosphoric acid in 100% acetonitrile in the reverse direction 3. 30 min water in the normal direction 4. Run analytical mobile phase overnight to lower the BGC. 5. Re-check the separation. |
Split peak or broadened peaks, shorter retention times for PP-ODS columns Print
Modified on: Fri, 4 Oct, 2019 at 2:21 PM
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