AC-GEL Wash Protocol:
Remove the enzyme column, and re-pack the precolumn. Collect wash solution directly from the column-end. NEVER pass effluent through the detector cell.
Wash as follows:
- With distilled water for 60 min at the standard flow rate and in the forward direction.
- With 100% methanol for 60 min at the standard flow rate reverse direction.
- With distilled water for 60 min at the standard flow rate and in the forward direction.
- Run mobile phase for 60 min at the standard flow rate in the forward direction
- Connect to enzyme column and detector cell
Although it is possible to analyze more than a few thousand microdialysis samples between cleanings, it is only possible to analyze a few hundred samples of tissue homogenize. Thus, cleaning cycles will depend on what kind of samples are used.
. Please ensure that the enzymatic column is detached as the organic solvent used to clean the column can permanently damage the enzyme activity.