Mobile Phase (flow rate 0.5 ml/min)

pH3.5 of 0.1M Citrate-Acetate Buffer* 83%

Methanol 17%

Sodium Octansulfonate (SOS) 190 mg/L

EDTA-2Na 5 mg/L

*0.1M pH 3.5 buffer can be prepared:

3.22 g Sodium acetate anhydrous + 9.17g Citric Acid monohydrous

total 830 mL with H2O.

Detection Condition

WE-3G, +750 mV vs Ag/AgCl.