Cells seeded onto the stretch chamber sometimes aggregate at the center of the chamber. Is there any technique to avoid this aggregation?

Derek Gonzales

Modified on: Thu, 19 Dec, 2019 at 10:42 AM

Cells may be migrating toward the center of the stretch chamber due to the vibration of the incubator. If that is the issue, seed the cells normally, then after 15 minutes, gently tilt the chamber from side to side.