How can cell proteins or mRNA be obtained from the Strex stretch chamber after culturing?

Derek Gonzales

Modified on: Thu, 19 Dec, 2019 at 10:41 AM

1. Western blotting: Wash with PBS, add electrophoresis sample buffer directly to the stretch chamber, then collect the cell lysate with a cell scraper.

2. Immunoprecipitation: Wash with PBS, add cell solubilizer directly to the stretch chamber, then collect the cell lysate with a cell scraper.

3. mRNA:Wash with PBS suitable for RNA, add cell solubilizer directly to the stretch chamber, then collect the cell lysate with a cell scraper.