Microdialysis

Antioxidant for catecholamines (norepinephrine, dopamine) and serotonin
20 mM Phosphate Buffer pH 3.5 (25 mM EDTA-2Na)*, before adding to microdialysate.  Mix ratio; [Antioxidant Solutiont] : [Microdialysis Sample] = 1: 1 to 4 ...
Tue, 16 Jul, 2019 at 2:47 PM
What is the number on the package for RT-5S tubing used for the ERP-10 roller pump?
The number on the package of the RT-5S roller pump tubing is the number setting for the ERP-10 roller pump to obtain a flow rate of 1 uL/min. Ex: **0022*...
Wed, 11 Sep, 2019 at 3:00 PM
How to calculate dead volume of microdialysis probes
The attached files will help you understand the dead volume of AZ, A-I, CX-I, C-I type probes. Please enter X (shaft length) and Y (membrane length) value t...
Thu, 18 Jul, 2019 at 2:08 PM
MI-AZ, MI-PEP, Injection Needle, Cannula Information
njection cannula position: We can make the injection cannula tip as you want. Either at the tip of membrane, at the middle of membrane or boarder between t...
Thu, 18 Jul, 2019 at 4:25 PM
Ringer's Solution, aCSF Composition, Recipe
The followings are recipe for the perfusate of microdialysis Eicom recommends. Do not use any buffer solutions as salts may precipitate and clog the probes....
Fri, 19 Jul, 2019 at 4:24 PM
Guide cannulae or implants falling off of the skull.
For implantation to be successful, the surface of the skull must be dry. If the surface is still damp when glue is applied, the trapped moisture will allow ...
Thu, 19 Dec, 2019 at 4:29 PM
Osmotic agents for preventing ultrafiltration during microdialysis
Dextran vs BSA: Most of our users add 0.15% serum albumin as an osmotic agent as opposed to dextran, for several reasons: -BSA is a better  blocking an...
Mon, 28 Oct, 2019 at 12:49 PM
Polyacrylonitrile membranes vs PES membranes
PAN membranes are negatively charged (sulfonate), while PES membranes have much less surface charge. My understanding is that PES membranes become fouled (b...
Mon, 28 Oct, 2019 at 12:49 PM
Protocol for making ACh standards
To prepare the 10 mM ACh standard solution, dissolve 245 mg acetylcholine perchlorate in 100 mL of 20 mM phosphate buffer, pH 3.5 (including 25 mM Na2EDTA...
Mon, 28 Oct, 2019 at 12:49 PM
What type of dental cement do you recommend to set microdialysis guide cannulae?
For mounting microdialysis guide cannulae to the skull, we recommend Geristore by DenMat https://www.denmat.com/geristore-a-paste-catalyst-jar.html  ht...
Wed, 13 Nov, 2019 at 1:18 PM