Solutions for Strex Cell Stretch Systems

Why does the culture medium evaporate after a few days while using a Strex STB-1400 stretching system?
The STB-1400 is not suitable for long-term culture, as it requires daily replenishment of culture fluid. We recommend using our stretching systems suitable ...
Thu, 19 Dec, 2019 at 10:41 AM
Will Strex silicone chambers degrade when we add reagents with phenols?
We use reagents with phenols on RNA extraction. How much will the silicone materials of the chambers degrade when we add the reagents directly?  We recomme...
Thu, 19 Dec, 2019 at 10:41 AM
Do you have any recommendation of gels to use for 3D cyclic cell stretching?
We recommend collagen gels for use in 3D cyclic stretching at this time. We have successfully used the following gel in house: KOKEN’s DME-02 Neutral collag...
Thu, 19 Dec, 2019 at 10:42 AM
Can we coat Strex stretch chambers under UV rays?
It is not recommended to coat chambers under UV rays. UV exposure after coating may denature proteins in the extracellular matrix on the chamber and may aff...
Thu, 19 Dec, 2019 at 10:42 AM
Cells adhesion appears to be different from chamber to chamber. What could be the Cause?
In producing the stretch chambers, STREX works to assure that there are no creases or other imperfections on any of the surfaces. However, given the excepti...
Thu, 19 Dec, 2019 at 10:42 AM
Do we have to hydrophilicate the silicone film before applying the coating agents?
STREX Stretch chambers are hydrophilicated with plasma processing before shipping.
Thu, 19 Dec, 2019 at 10:42 AM
How do you confirm the completion of coating?
Some cells adhere without coating. For example, some cells secrete collagens, such as smooth muscle cells and fibroblasts. You can check the degree of coati...
Thu, 19 Dec, 2019 at 10:42 AM
Do we have to air-dry after four-hour standing?
We coat with fibronectin just before using and have never done air-drying. However, some users have reported air-drying after coating with collagen.
Thu, 19 Dec, 2019 at 10:42 AM
Do you coat with fibronectins without pretreatment on the methyl groups on the surface?
We do not recommend conducting any treatments on the surface of the chambers. Some chamber treatment during the manufacturing process such as plasma process...
Thu, 19 Dec, 2019 at 10:42 AM
Which extracellular matrix works best for cell adhesion of the Strex stretch chambers? Fibronectin, gelatin, or collagen?
Cell adhesion to the stretch chamber membrane is entirely dependent on the cells bonding to the extracellular matrix coating. The cells themselves vary in a...
Thu, 19 Dec, 2019 at 10:42 AM