Solutions for Strex Cell Stretch Systems

Can we load shear stress on cells using the Strex cell stretch system?
The Strex cell stretch system is intended to work with adhesive cells, therefore it is difficult to load shear stress with this system.
Thu, 19 Dec, 2019 at 10:41 AM
Can we stretch cells using the Strex cell stretch device undergoing gene transfer?
You can conduct gene transfer directly on stretch chambers. However, the transfer work damages cells so that it becomes difficult to snap images of high qua...
Thu, 19 Dec, 2019 at 10:41 AM
How can cell proteins or mRNA be obtained from the Strex stretch chamber after culturing?
1. Western blotting: Wash with PBS, add electrophoresis sample buffer directly to the stretch chamber, then collect the cell lysate with a cell scraper. ...
Thu, 19 Dec, 2019 at 10:41 AM
Is there an assay to measure tension load for fibroblasts after stretching with the Strex cell stretch system?
We recommend phosphorylation of ERK with Western Blotting.
Thu, 19 Dec, 2019 at 10:41 AM
Could you show any report saying the amount of mRNA in gene of mechanosensitive channels changes drastically under stretching stimulation using the Strex cell stretch system?
Some genes in mechanosensitive channels are well known, however many of them have not been identified yet. So we cannot show the references on this topic at...
Thu, 19 Dec, 2019 at 10:41 AM
Is there any report saying the amount of β-actin in cells is changed under stresses?
Amount of β-actin in cells is not considered to change so drastically under stresses, except for long-term experiments.
Thu, 19 Dec, 2019 at 10:41 AM
Can we conduct fluorescent staining and observations with the Strex membrane stretched?
Yes. Follow the protocol in the manual or use our trial kit with a stretching stand.
Thu, 19 Dec, 2019 at 10:41 AM
We are planning to stretch the cells on chambers to observe them after fixation and staining. Do the Strex stretch chambers degrade on the fixation and staining?
If you use acetone or chloroform, the silicone film of chambers may swell a little. You have no problems with methanol. Tip: Splicing the silicone chamber f...
Thu, 19 Dec, 2019 at 10:41 AM
Can we use immersion lens in the observation of fixed and fluorescent-stained cells after stretching with the Strex cell stretch system?
You can use immersion lens. However, the choice of chamber is limited in accordance with the size of the object lens to use.
Thu, 19 Dec, 2019 at 10:41 AM
The microscope observations of cells using the Strex cell stretch system appear out of focus.
Do you use oil-immersion lens? The existing PDMS films swell when they are exposed on oils and make the observation difficult. We recommend using water-imme...
Thu, 19 Dec, 2019 at 10:41 AM