Solutions for Strex Cell Stretch Systems
The Strex cell stretch system is intended to work with adhesive cells, therefore it is difficult to load shear stress with this system.
Thu, 19 Dec, 2019 at 10:41 AM
You can conduct gene transfer directly on stretch chambers. However, the transfer work damages cells so that it becomes difficult to snap images of high qua...
Thu, 19 Dec, 2019 at 10:41 AM
1. Western blotting: Wash with PBS, add electrophoresis sample buffer directly to the stretch chamber, then collect the cell lysate with a cell scraper. ...
Thu, 19 Dec, 2019 at 10:41 AM
We recommend phosphorylation of ERK with Western Blotting.
Thu, 19 Dec, 2019 at 10:41 AM
Some genes in mechanosensitive channels are well known, however many of them have not been identified yet. So we cannot show the references on this topic at...
Thu, 19 Dec, 2019 at 10:41 AM
Amount of β-actin in cells is not considered to change so drastically under stresses, except for long-term experiments.
Thu, 19 Dec, 2019 at 10:41 AM
Yes. Follow the protocol in the manual or use our trial kit with a stretching stand.
Thu, 19 Dec, 2019 at 10:41 AM
If you use acetone or chloroform, the silicone film of chambers may swell a little. You have no problems with methanol. Tip: Splicing the silicone chamber f...
Thu, 19 Dec, 2019 at 10:41 AM
You can use immersion lens. However, the choice of chamber is limited in accordance with the size of the object lens to use.
Thu, 19 Dec, 2019 at 10:41 AM
Do you use oil-immersion lens? The existing PDMS films swell when they are exposed on oils and make the observation difficult. We recommend using water-imme...
Thu, 19 Dec, 2019 at 10:41 AM