There are three possible causes: 1. Cell density The cell concentration of the culture may be too dense. Generally speaking, over-confluence will cause the...

Cells may be migrating toward the center of the stretch chamber due to the vibration of the incubator. If that is the issue, seed the cells normally, then a...

Rounded cells are generally more difficult to be stretched in comparison with flat-shaped cells.

Cells in the incubator can be stretched for a period of two weeks. However, in these long-term experiments, the culture fluid has to be replaced at 2-3 day ...

In general, 200,000 cells / chamber (4 mL of culture medium) is proper from our experience.

We recommend coating Strex cell stretch chambers with the extracellular matrix at room temperature.

We do not foresee any problems with laminin coating, but unfortunately we do not have any experience using laminin coating.

As a general guide, we say that the cells of 5 × 104 cm2 are adequate. However, this can vary depending on experimental parameters.

Unfortunately no movie is available. Please refer to the following paper for photos of fluorescent images of GFP-actin transferred cells in the stretched st...

Yes. Please see this reference.