HTEC-500/510

Split peak or broadened peaks, PP-ODS columns
Problem: Broadened or split peaks were observed on chromatogram of PP-ODS2. Dopamine seemed OK, but if magnified the peak was broadened as well. The retenti...
Fri, 19 Jul, 2019 at 4:28 PM
Best urine sample storage for catecholamine analysis?
: add equal volumes of 6 N HCl including 100 µM EDTA and urine then store at 4ºC. Add ammonia solution and titrate the pH to 4-7 before processing with the ...
Fri, 19 Jul, 2019 at 4:30 PM
What concentration of standard solutions should I inject?
 Inject standards at the concentration(s) around where your sample concentrations are expected to be before, after, or between samples everyday.  Standards ...
Fri, 4 Oct, 2019 at 2:22 PM
What is a normal pump pressure reading for each column?
Please note there are many variables that can change the pressure such as tubing length, temperature, solvent composition, and precolumn.  There is some bac...
Thu, 9 Jul, 2020 at 1:45 PM
Where should I place precolumn. What type of precolumn should I use?
The precolumn is most beneficial when placed before the separation column. This placement works most efficiently to remove contaminants from both the mobil...
Fri, 19 Jul, 2019 at 4:37 PM
Why are the tops of my peaks cut off?
When the tops of peaks appear flat, the data above the point has been lost. The Data Acquisition parameters needs to be set to a higher value. In Clarity/E...
Fri, 4 Oct, 2019 at 2:24 PM
Why is my baseline noisy? (Baseline means the signal from the ECD, not animal response)
Why is my baseline noisy? Many things can contribute to a noisy baseline ECD reference electrode needs to be new and not dry. If the reference electrod...
Fri, 19 Jul, 2019 at 4:39 PM
9725i manual Injector handle loose, injector is leaking
The instructions for adjusting the injector for leakage are in section 9 and fig. 9 of the attached manual. When reattaching the handle after the adjustment...
Thu, 26 Sep, 2019 at 2:57 PM
Pulsatile flow is found on the baseline of the HTEC
Possible causes are as follows: There is a leakage from the flow line. Columns are not held by column clips properly to ground static electrical cur...
Fri, 4 Oct, 2019 at 2:01 PM
High Background Current
If the Background Current (BGC) is too high, possible reasons include the following: If the separation column and/or precolumn are new, please wash the c...
Fri, 4 Oct, 2019 at 2:05 PM