HTEC-500/510

Big peak before GABA peak
At pH2.8 condition for GABA analysis, you will see a very large peak in front of the GABA peak. If the separation of those two peaks are not good, please ra...
Fri, 4 Oct, 2019 at 2:10 PM
No peak on the HTEC-500, ECD
To confirm the system sensitivity, use the manual injector first.  If this is an issue of the autosampler, please see the autosampler manual.   1.      En...
Fri, 12 Feb, 2021 at 8:32 AM
How to remove the pump unit from the HTEC-500/510
Please contact Amuza before removing or shipping any HTEC parts.   -Please purge the pump with water. - Please remove PEEK tubing with nuts, four piston ...
Fri, 4 Oct, 2019 at 2:11 PM
Split peak or broadened peaks, shorter retention times for PP-ODS columns
PP-ODS2 column needs to be washed. If the standard protocol as outlined in the user manual doesn’t solve the problem, proceed to wash the column as foll...
Fri, 4 Oct, 2019 at 2:21 PM
L-DOPA Analysis Condition
System; HTEC-500/510  Sample; Tissue Homogenate  Column; Eicompak SC-5ODS  Mobile Phase Condition; 15% MeOH 85% 0.1 M Citrate-Acetate Buffer pH2.6* 110 mg/l ...
Thu, 18 Jul, 2019 at 4:24 PM
No flow at all from HTEC-500/510 pump
If the pump pressure reading is zero or nearly zero, there is a possibility that the tubing or degasser located upstream of the HPLC pump has been clogged. ...
Fri, 4 Oct, 2019 at 2:12 PM
High Noise with acetylcholine or glutamate application
If you are experiencing high noise with acetylcholine or glutamate applications, please ensure the enzyme reactor is properly grounded using the grounding c...
Fri, 4 Oct, 2019 at 2:12 PM
Pump pressure is high
High pressure is often caused by system clogging.  To identify the part that is the problem, remove each flow line at the connection fitting from downstrea...
Fri, 4 Oct, 2019 at 2:13 PM
Pump pressure is low
The most frequent trouble with the pump is low pressure. The pump of the HTEC-500 does not pump out air and a buildup of air will result and prevent pressure...
Fri, 19 Jul, 2019 at 4:20 PM
RE-500 Reference Electrode Storage
Submerge the reference electrode (RE-500) tip in 1M LiCl diluted in 0.1M CH3COOH/water and seal to prevent evaporation. Then it can be stored for one year. ...
Fri, 19 Jul, 2019 at 4:22 PM