HTEC-500/510

Norepinephrine and epinephrine separation
The EICOMPAK CAX with the current separation conditions does not allow the complete separation of NE and epinephrine. The difference in their retention time...
Mon, 28 Oct, 2019 at 12:48 PM
Pressure drops to zero on the HTEC without a pump error
 If there is a condition in which there is a gradual change in pressure (ex. gradually down to zero), the pump may stop working without throwing any errors....
Mon, 28 Oct, 2019 at 12:50 PM
What is the Lifespan of the Acetylcholine (AC-GEL) Column?
We recommend washing the AC-GEL column every 3-4 months during periods of normal use with microdialysis samples, but much more frequently if analyzing tissu...
Mon, 28 Oct, 2019 at 12:50 PM
Wash protocol for Acetylcholine (AC-GEL) Column
AC-GEL Wash Protocol:  Remove the enzyme column, and re-pack the precolumn. Collect wash solution directly from the column-end. NEVER pass effluent thro...
Wed, 15 Jan, 2020 at 3:57 PM
PEEK Tubing Volume/Length Conversion Chart By Color
Mon, 28 Oct, 2019 at 12:50 PM
Care and Use of Electrodes
Electrode Care Procedures Standard Cleaning Cleaning the electrode periodically will help to recover lost sensitivity. If the same amount of fresh stan...
Wed, 30 Oct, 2019 at 9:28 AM
How often should I repack the precolumn?
A pre-column is applied to the system to avoid the contamination of a separation column by protein etc. The lifetime of a separation column depends on a sta...
Tue, 2 Feb, 2021 at 9:38 AM
I cannot pull water through the washline of the HTEC
"I cannot pull water through the wash line. It moves a bit but there is very little resistance and it doesn't draw water into the syringe." C...
Fri, 8 Nov, 2019 at 11:04 AM
How much lab space is required for the HTEC?
The HTEC requires a benchtop space at least: 0.5 meters wide without the autosampler 1 meter wide with the autosampler
Tue, 12 Nov, 2019 at 9:22 AM
What kind of water is suitable for running HPLC-ECD Analysis?
Use ultrapure water (the resistance of the water needs to be at least 18.2 mega-ohm/cm). Any solvent, such as mobile phase, or water applied to this system...
Thu, 19 Dec, 2019 at 10:42 AM